Immunohistochemical characterisation of Fos-positive cells in brainstem monoaminergic nuclei following intracranial self-stimulation of the medial forebrain bundle in the rat

Authors

  • Yasushi Ishida,

    1. 1 Department of Psychiatry, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 2 Division of Biology, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 3 Department of Psychology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu 431–3192, Japan 4 Department of Neurobiology and Anatomy, Niigata University School of Medicine, Niigata 951–8510, Japan
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  • 1 Masato Nakamura,

    1. 1 Department of Psychiatry, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 2 Division of Biology, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 3 Department of Psychology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu 431–3192, Japan 4 Department of Neurobiology and Anatomy, Niigata University School of Medicine, Niigata 951–8510, Japan
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  • 3 Kosuke Ebihara,

    1. 1 Department of Psychiatry, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 2 Division of Biology, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 3 Department of Psychology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu 431–3192, Japan 4 Department of Neurobiology and Anatomy, Niigata University School of Medicine, Niigata 951–8510, Japan
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  • 3 Kaeko Hoshino,

    1. 1 Department of Psychiatry, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 2 Division of Biology, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 3 Department of Psychology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu 431–3192, Japan 4 Department of Neurobiology and Anatomy, Niigata University School of Medicine, Niigata 951–8510, Japan
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  • 4 Hiroyuki Hashiguchi,

    1. 1 Department of Psychiatry, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 2 Division of Biology, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 3 Department of Psychology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu 431–3192, Japan 4 Department of Neurobiology and Anatomy, Niigata University School of Medicine, Niigata 951–8510, Japan
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  • 1 Yoshio Mitsuyama,

    1. 1 Department of Psychiatry, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 2 Division of Biology, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 3 Department of Psychology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu 431–3192, Japan 4 Department of Neurobiology and Anatomy, Niigata University School of Medicine, Niigata 951–8510, Japan
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  • 1 Toshikazu Nishimori,

    1. 1 Department of Psychiatry, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 2 Division of Biology, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 3 Department of Psychology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu 431–3192, Japan 4 Department of Neurobiology and Anatomy, Niigata University School of Medicine, Niigata 951–8510, Japan
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  • and 2 Daiichiro Nakahara 3

    1. 1 Department of Psychiatry, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 2 Division of Biology, Miyazaki Medical College, 5200 Kihara, Kiyotake-cho, Miyazaki 889–1692, Japan 3 Department of Psychology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu 431–3192, Japan 4 Department of Neurobiology and Anatomy, Niigata University School of Medicine, Niigata 951–8510, Japan
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: Dr Y. Ishida, as above.
E-mail: ishiday@post.miyazaki-med.ac.jp

Abstract

Fos immunostaining was used as a marker of neuronal activity following intracranial self-stimulation (ICSS) of the medial forebrain bundle (MFB) in the rat, and was combined with immunostaining for tyrosine hydroxylase (TH), serotonin (5-HT), γ-aminobutyric acid (GABA), or NR1 (one of the glutamate N-methyl-d-aspartate receptor subunits) for purposes of neurochemical identification. ICSS induced a significant but different degree of increase in the number of Fos-immunopositive (Fos+) cells in the six brainstem monoaminergic nuclei examined, which included the ventral tegmental area (VTA), substantia nigra pars compacta (SNc), dorsal raphe nucleus (DR), median raphe nucleus (MR), locus coeruleus (LC), and A7 noradrenaline cells. Densely labelled Fos+ cells were observed in the LC following ICSS, and many of these Fos+ cells were colocalized with TH. Similarly, many of Fos+ cells in the A7 and DR/MR were colocalized with TH and 5-HT, respectively. By contrast, a smaller number of Fos+ cells was detected in the VTA and SNc following the ICSS, and in these regions the majority of Fos+ cells were not colocalized with TH. Although results among regions quantitatively differed, the ICSS induced a significant increase in the number of double-labelled cells (GABA+/Fos+ or NR1+/Fos+) in all of the VTA, DR, and LC, in which the ICSS produced an ipsilaterally weighted increase in Fos-like immunoreactivity. These results suggest that ICSS of the MFB induces differential Fos expression within monoaminergic and GABAergic neurons in brainstem monoaminergic nuclei under modulation by glutamatergic afferents.

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