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Gene expression profiles during long-term memory consolidation

Authors

  • Sebastiano Cavallaro,

    1. 1Laboratory of Adaptive Systems, NINDS, NIH, Bethesda, MD 20892, USA
    2. Institute of Bioimaging and Pathophysiology of the Central Nervous System, CNR, 95123 Catania, Italy
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  • Bernard G. Schreurs,

    1. 1Laboratory of Adaptive Systems, NINDS, NIH, Bethesda, MD 20892, USA
    2. Blanchette Rockefeller Neurosciences Institute, Rockville, MD 20850, USA
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    • *

      Present address: BRNI and Dept. Physiology, West Virginia University, Morgantown, WV 26506, USA.

  • Weiqin Zhao,

    1. 1Laboratory of Adaptive Systems, NINDS, NIH, Bethesda, MD 20892, USA
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  • Velia D'Agata,

    1. 1Laboratory of Adaptive Systems, NINDS, NIH, Bethesda, MD 20892, USA
    2. Institute of Bioimaging and Pathophysiology of the Central Nervous System, CNR, 95123 Catania, Italy
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  • Daniel L. Alkon

    1. 1Laboratory of Adaptive Systems, NINDS, NIH, Bethesda, MD 20892, USA
    2. Department of Neurology, West Virginia University, Morgantown, WV 26056, USA
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: Dr S. Cavallaro, Blanchette Rockefeller Neurosciences Institute, Academic and Research Building, Room 315, 9601 Medical Center Drive, Rockville, MD 20850, USA
E-mail: sebi@brni-jhu.org

Abstract

Changes in gene expression have been postulated to occur during long-term memory (LTM). We used high-density cDNA microarrays to assess changes in gene expression 24 h after rabbit eye blink conditioning. Paired animals were presented with a 400 ms, 1000 Hz, 82 dB tone conditioned stimulus that coterminated with a 100 ms, 60 Hz, 2 mA electrical pulse unconditioned stimulus. Unpaired animals received the same conditioned and unconditioned stimuli but presented in an explicitly unpaired manner. Differences in expression levels between paired and unpaired animals in the hippocampus and cerebellar lobule HVI, two regions activated during eye blink conditioning, indicated the involvement of novel genes as well as the participation of previously implicated genes. Patterns of gene expression were validated by in situ hybridization. Surprisingly, the data suggest that an underlying mechanism of LTM involves widespread decreased, rather than increased, gene expression. These results demonstrate the feasibility and utility of a cDNA microarray system as a tool for dissecting the molecular mechanisms of associative memory.

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