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Independent receptors for diadenosine pentaphosphate and ATP in rat midbrain single synaptic terminals

Authors

  • M. Díaz-Hernández,

    1. Departamento de Bioquímica y Biología Molecular IV, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
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  • J. Pintor,

    1. Departamento de Bioquímica y Biología Molecular IV, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
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  • E. Castro,

    1. Departamento de Bioquímica y Biología Molecular IV, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
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  • M. T. Miras-Portugal

    1. Departamento de Bioquímica y Biología Molecular IV, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
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: Professor M. T. Miras-Portugal, as above.
E-mail: mtmiras@vet.ucm.es

Abstract

Diadenosine pentaphosphate (Ap5A) and adenosine 5′-triphosphate (ATP) stimulate a intrasynaptosomal calcium concentration [Ca2+]i increase via specific purinergic receptors in rat midbrain synaptosomes, although nothing is known about their distribution in presynaptic terminals. A microfluorimetric technique to measure [Ca2+]i increase using the dye FURA-2AM, has permitted study of the presence of dinucleotide and P2X receptors in independent isolated synaptic terminals. Our results demonstrate the existence of three populations of synaptosomes: one with dinucleotide receptors (12%), another with P2X receptors (20%) and a third with both (14%). It has been possible to demonstrate that the activation of these receptors occurs only in the presence of extracellular Ca2+ and that it is also coupled with voltage-dependent Ca2+ channels. Finally 54% of the synaptosomes that responded to K+ did not present any calcium increase mediated by the nucleotides used. In summary, ATP and dinucleotides exhibit specific ionotropic receptors that can coexist or not on the same synaptic terminal.

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