Temporally controlled targeted somatic mutagenesis in the mouse brain

Authors

  • Philipp Weber,

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      Present address: Brain Research Institute, University and ETH Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland

  • Daniel Metzger,

    1. Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Collège de France, Université Louis Pasteur, BP163, 67404 Illkirch-Cedex, Communauté Urbaine de Strasbourg, France
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  • Pierre Chambon

    1. Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Collège de France, Université Louis Pasteur, BP163, 67404 Illkirch-Cedex, Communauté Urbaine de Strasbourg, France
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: Professor Pierre Chambon, as above.
E-mail: chambon@igbmc.u-strasbg.fr

Abstract

To develop spatio-temporally controlled somatic mutagenesis in the adult mouse nervous system, we established transgenic mice expressing the tamoxifen-inducible Cre-ERT recombinase under the control of the mouse prion protein (PrP) promoter. Cre-ERT was expressed in most regions of the brain and in the retina of one transgenic line, whereas its expression was mostly restricted to the hippocampus and the cerebellum in another line. As tamoxifen efficiently induced Cre-mediated recombination in the various neuronal cell types expressing Cre-ERT in the brain of adult mice, the PrP-Cre-ERT lines should be valuable tools to study the functions of genes involved in neurodegenerative diseases or regeneration, and in complex processes such as behaviour, learning and memory. Some limitations of presently available reporter lines for Cre-mediated recombination in adult mouse CNS are discussed.

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