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Use of computerized image analysis to quantify staphylococcal adhesion to canine corneocytes: does breed and body site have any relevance to the pathogenesis of pyoderma?

Authors

  • Peter J. Forsythe,

    Corresponding author
    1. The University of Edinburgh, Department of Veterinary Clinical Studies, The Royal (Dick) School of Veterinary Studies, Hospital for Small Animals, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK
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  • Peter B. Hill,

    1. The University of Edinburgh, Department of Veterinary Clinical Studies, The Royal (Dick) School of Veterinary Studies, Hospital for Small Animals, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK
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  • Keith L. Thoday,

    1. The University of Edinburgh, Department of Veterinary Clinical Studies, The Royal (Dick) School of Veterinary Studies, Hospital for Small Animals, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK
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  • Jeremy Brown

    1. The University of Edinburgh, Department of Veterinary Clinical Studies, The Royal (Dick) School of Veterinary Studies, Hospital for Small Animals, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK
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Peter J. Forsythe, Lugton Farm, Aiket Road, Dunlop KA3 4BP, UK. E-mail: pforsythe@vetderm.demon.co.uk

Abstract

An optimized system of computerized image analysis was used to investigate variations in the adherence of Staphylococcus intermedius to canine corneocytes from four different breed groups and six different anatomical sites. S. intermedius showed significantly greater adherence to the head and neck compared with the dorsum, but adherence to the limb, axilla and groin did not differ from other sites. Furthermore, there was significantly greater adherence of S. intermedius to corneocytes from the dorsum, forelimb, axilla and groin of Boxers and Bull Terriers than Spaniels and Hounds. S. intermedius, and also Pseudomonas aeruginosa, exhibited abundant adherence, which was significantly greater than Staphylococcus aureus, Streptococcus canis, Klebsiella pneumoniae and Escherichia coli. In addition, S. intermedius adherence demonstrated a sigmoid dose–response curve with increasing bacterial concentration. These results suggest that S. intermedius adheres to canine corneocytes by a specific receptor–ligand interaction and adheres to the skin of some breeds more avidly than others. However, variations in adherence between body regions would not account for the predilection sites of canine bacterial pyoderma.

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