These authors contributed equally to this work.
Technical Advance: Tobacco rattle virus as a vector for analysis of gene function by silencing
Article first published online: 18 JUL 2008
The Plant Journal
Volume 25, Issue 2, pages 237–245, January 2001
How to Cite
Ratcliff, F., Martin-Hernandez, A. M. and Baulcombe, D. C. (2001), Technical Advance: Tobacco rattle virus as a vector for analysis of gene function by silencing. The Plant Journal, 25: 237–245. doi: 10.1046/j.0960-7412.2000.00942.x
- Issue published online: 18 JUL 2008
- Article first published online: 18 JUL 2008
- Received 11 August 2000; revised 18 October 2000; accepted 23 October 2000.
- gene silencing;
- virus vector;
- phytoene desaturase;
- green fluorescent protein
Virus vectors carrying host-derived sequence inserts induce silencing of the corresponding genes in infected plants. This virus-induced gene silencing (VIGS) is a manifestation of an RNA-mediated defence mechanism that is related to post-transcriptional gene silencing (PTGS) in transgenic plants. Here we describe an infectious cDNA clone of tobacco rattle virus (TRV) that has been modified to facilitate insertion of non-viral sequence and subsequent infection to plants. We show that this vector mediates VIGS of endogenous genes in the absence of virus-induced symptoms. Unlike other RNA virus vectors that have been used previously for VIGS, the TRV construct is able to target host RNAs in the growing points of plants. These features indicate that the TRV vector will have wide application for gene discovery in plants.