Golgi secretion is not required for marking the preprophase band site in cultured tobacco cells
Article first published online: 11 JAN 2002
The Plant Journal
Volume 29, Issue 1, pages 99–108, January 2002
How to Cite
Dixit, R. and Cyr, R. (2002), Golgi secretion is not required for marking the preprophase band site in cultured tobacco cells. The Plant Journal, 29: 99–108. doi: 10.1046/j.0960-7412.2001.01202.x
- Issue published online: 11 JAN 2002
- Article first published online: 11 JAN 2002
- Received 23 July 2001; revised 10 October 2001; accepted 17 October 2001.
- preprophase band;
- Golgi stacks;
- brefeldin A;
- green fluorescent protein;
- red fluorescent protein
The preprophase band predicts the future cell division site. However, the mechanism of how a transient preprophase band fulfils this function is unknown. We have investigated the possibility that Golgi secretion might be involved in marking the preprophase band site. Observations on living BY-2 cells labeled for microtubules and Golgi stacks indicated an increased Golgi stack frequency at the preprophase band site. However, inhibition of Golgi secretion by brefeldin A during preprophase band formation did not prevent accurate phragmoplast fusion, and subsequent cell plate formation, at the preprophase band site. The results show that Golgi secretion does not mark the preprophase band site and thus does not play an active role in determination of the cell division site.