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Using the δ13C and δ15N of whitefish scales for retrospective ecological studies: changes in isotope signatures during the restoration of Lake Geneva, 1980–2001

Authors

  • M. E. Perga,

    Corresponding author
    1. Station d’Hydrobiologie Lacustre, INRA, 75, avenue de Corzent, BP 511, 74203 Thonon les Bains Cedex, France
      *Tel.: +33 450 26 78 32; fax: +33 450 26 07 60; email: perga@thonon.inra.fr
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  • D. Gerdeaux

    1. Station d’Hydrobiologie Lacustre, INRA, 75, avenue de Corzent, BP 511, 74203 Thonon les Bains Cedex, France
    Search for more papers by this author

*Tel.: +33 450 26 78 32; fax: +33 450 26 07 60; email: perga@thonon.inra.fr

Abstract

The scales of whitefish Coregonus lavaretus were used in place of dorsal muscle, which necessitates killing the fish, to study food webs from the δ13C and δ15N isotopic ratios in the organic fraction. As scales are composed of both organic and calcified fractions, a protocol for scale decalcification was first devised. The δ13C and δ15N values of the decalcified scales were then shown to be closely correlated to those of the dorsal muscle, demonstrating that scales could be used in place of muscle to study food webs. Changes in the δ13C of whitefish were determined from a scale collection that extended over the period during which the trophic state of Lake Geneva was recovering.

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