Present addresses: CropDesign NV, Technologiepark 3, B-9052 Gent, Belgium; ‡Vakgroep Moleculaire Biotechnologie, Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen, Universiteit Gent, Coupure links 653, B-9000 Gent, Belgium.
Identification of cytokinins produced by the plant parasitic nematodes Heterodera schachtii and Meloidogyne incognita
Article first published online: 10 JUL 2003
Molecular Plant Pathology
Volume 4, Issue 4, pages 271–277, July 2003
How to Cite
De Meutter, J., Tytgat, T., Witters, E., Gheysen, G., Van Onckelen, H. and Gheysen, G. (2003), Identification of cytokinins produced by the plant parasitic nematodes Heterodera schachtii and Meloidogyne incognita. Molecular Plant Pathology, 4: 271–277. doi: 10.1046/j.1364-3703.2003.00176.x
- Issue published online: 10 JUL 2003
- Article first published online: 10 JUL 2003
The presence of different types of cytokinins was analysed in exudates and lysates of stage-2 juveniles of Heterodera schachtii and Meloidogyne incognita and in mixed stages of Caenorhabditis elegans. For all species, cytokinins were detected in lysates and exudates in which benzyladenine and zeatin-type cytokinins were the most prominent forms. The production of cytokinins by Meloidogyne was much higher than by Heterodera, and the detected levels were in a range which interfered with the physiological activities of the host plant. The presence of 5-methoxy-N,N-dimethyltryptamine hydrogen oxalate did not affect hormone production by H. schachtii, whereas resorcinol slightly stimulated hormone production by M. incognita. The exuded cytokinins may play a role in feeding site induction, more particularly in cell cycle activation and in establishing the feeding site as a nutrient sink.