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The Arabidopsis mutant iop1 exhibits induced over-expression of the plant defensin gene PDF1.2 and enhanced pathogen resistance

Authors

  • Iris A. M. A. Penninckx,

    1. Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee-Leuven, Belgium;
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  • Kristel Eggermont,

    1. Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee-Leuven, Belgium;
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  • Peer M. Schenk,

    1. Cooperative Research Centre for Tropical Plant Protection, University of Queensland, St Lucia, Australia;
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  • Guido Van den Ackerveken,

    1. Department of Molecular and Cellular Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands
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  • Bruno P. A. Cammue,

    1. Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee-Leuven, Belgium;
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  • Bart P. H. J. Thomma

    Corresponding author
    1. Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee-Leuven, Belgium;
      *Correspondence: Bart P.H.J. Thomma, Laboratory of Phytopathology, Wageningen University, PO Box 8025, 6700 EE Wageningen, the Netherlands. Tel.: +31 317 484536, Fax: +31 317 483412, E-mail: bart.thomma@wur.nl
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    • Present address: Laboratory of Phytopathology, Wageningen University, Binnenhaven 5, 6709 PD Wageningen, the Netherlands.


*Correspondence: Bart P.H.J. Thomma, Laboratory of Phytopathology, Wageningen University, PO Box 8025, 6700 EE Wageningen, the Netherlands. Tel.: +31 317 484536, Fax: +31 317 483412, E-mail: bart.thomma@wur.nl

SUMMARY

Jasmonate and ethylene are concomitantly involved in the induction of the Arabidopsis plant defensin gene PDF1.2. To define genes in the signal transduction pathway leading to the induction of PDF1.2, we screened for mutants with induced over-expression of a β-glucuronidase reporter, under the control of the PDF1.2 promoter. One mutant, iop1 (induced over-expressor of PDF1.2) produced small plants that showed induced over-expression of the pathogenesis-related genes PR-3, PR-4 and PR-1,2 (PDF1.2), combined with a down-regulated induction of PR-1 upon pathogen inoculation. The iop1 mutant showed enhanced resistance to a number of necrotrophic pathogens.

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