High-resolution gametic map of the sheep callipyge region: linkage heterogeneity among rams detected by sperm typing

  1. S. Lien,
  2. N. E. Cockett,
  3. H. Klungland,
  4. N. Arnheim,
  5. M. Georges,
  6. L. Gomez-Raya

Article first published online: 24 DEC 2001

DOI: 10.1046/j.1365-2052.1999.00430.x

Issue

Animal Genetics

Animal Genetics

Volume 30, Issue 1, pages 42–46, February 1999

Additional Information

How to Cite

Lien, S., Cockett, N. E., Klungland, H., Arnheim, N., Georges, M. and Gomez-Raya, L. (1999), High-resolution gametic map of the sheep callipyge region: linkage heterogeneity among rams detected by sperm typing. Animal Genetics, 30: 42–46. doi: 10.1046/j.1365-2052.1999.00430.x

Author Information

*L. Gomez-Raya Department of Animal Science, Agricultural University of Norway, 1432 Aas, Norway N. E. Cockett Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322–4700, USA N. Arnheim Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089–1340, USA M. Georges Department of Genetics, Faculty of Veterinary Medicine, University of Liège (B43), 20 Bd de Colonster, 4000-Liège, Belgium

Publication History

  1. Issue published online: 24 DEC 2001
  2. Article first published online: 24 DEC 2001
  3. Accepted 6 November 1998

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Keywords:

  • callipyge locus;
  • linkage heterogeneity;
  • sperm typing

The callipyge locus (CLPG) causing muscular hypertrophy in domestic sheep has previously been mapped to the distal part of ovine chromosome 18. In this study, an accurate multipoint linkage map consisting of six microsatellite markers in this chromosomal region was constructed based on the analysis of 1145 single sperm cells. The best supported order of markers was OARHH47-ILSTS54-MCM38-CSSM18-IDVGA30-BMS1561. The log odds against the second most likely order, which has a reversal of the closely linked markers CSSM18 and IDVGA30, was 5·026. Sperm typing can be used to examine a large number of meioses in single individuals, and therefore, was exploited to study individual variability of recombination rate in rams of different callipyge genotypes. The results revealed statistically significant linkage heterogeneity among rams (P < 0·05) for marker interval OARHH47-CSSM18, with individual recombination fractions varying from 0·209 to 0·357.

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