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Applicability of bovine microsatellite markers for population genetic studies on African buffalo (Syncerus caffer)
Article first published online: 24 DEC 2001
DOI: 10.1046/j.1365-2052.1999.00453.x
1365-2052/asset/cover.gif?v=1&s=471293ae73aa616ae6db698a4e5ccacf1b977787 "Animal Genetics")
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How to Cite
Van Hooft, W. F., Hanotte, O., Wenink, P. W., Groen, A. F., Sugimoto, Y., Prins, H. H. T. and Teale, A. (1999), Applicability of bovine microsatellite markers for population genetic studies on African buffalo (Syncerus caffer). Animal Genetics, 30: 214–220. doi: 10.1046/j.1365-2052.1999.00453.x
Publication History
- Issue published online: 24 DEC 2001
- Article first published online: 24 DEC 2001
- Abstract
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Keywords:
- African buffalo;
- bovine;
- interspecific amplification;
- microsatellites;
- polymorphism;
- Syncerus caffer
The applicability of bovine autosomal microsatellite markers for population genetic studies on African buffalo was investigated. A total of 168 microsatellite markers were tested for PCR amplification on a test panel of seven African buffalo. Amplification was observed for 139 markers (83%), and 101 markers were studied further with 91 (90%) being polymorphic. The mean number of alleles per marker was 5·0 (SE= 0·2) and the mean heterozygosity per marker was 0·61 (SE= 0·03). Considering the overall high level of polymorphism, it was concluded that most bovine microsatellite markers are applicable in African buffalo.