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Characterization of two new alleles at the goat CSN1S2 locus


L. Ramunno, Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Università degli Studi di Napoli ‘Federico II’, Via Università 133, 80055 Portici (Na), Italy. E-mail:


Two novel alleles at the goat CSN1S2 locus have been identified: CSN1S2F and CSN1S2D. Sequence analyses revealed that the CSN1S2F allele is characterized by a G → A transition at the 13th nucleotide in exon 3 changing the seventh amino acid of the mature protein from Val to Ile. The CSN1S2D allele, apparently associated with a decreased synthesis of αs2-casein, is characterized by a 106-bp deletion, involving the last 11 bp of the exon 11 and the first 95 bp of the following intron. Methods (PCR–RFLP and PCR) for identification of carriers of these alleles have been developed.