Melanoma cell-derived factors stimulate glycosaminoglycan synthesis by fibroblasts cultured as monolayers and within contracted collagen lattices
Article first published online: 23 DEC 2001
British Journal of Dermatology
Volume 144, Issue 3, pages 465–470, February 2001
How to Cite
Edward, M. (2001), Melanoma cell-derived factors stimulate glycosaminoglycan synthesis by fibroblasts cultured as monolayers and within contracted collagen lattices. British Journal of Dermatology, 144: 465–470. doi: 10.1046/j.1365-2133.2001.04069.x
- Issue published online: 23 DEC 2001
- Article first published online: 23 DEC 2001
- Accepted for publication 24 October 2000
- collagen lattices;
Background Various tumours exhibit glycosaminoglycan rich, and in particular hyaluronan rich matrices surrounding them that facilitate tumour growth and invasion. In many tumours, this matrix is predominantly synthesized by fibroblasts following stimulation by tumour cell-derived factors.
Objectives To determine what effect tumour cell-conditioned medium has upon fibroblast glycosaminoglycan synthesis when cells were cultured as monolayers and within contracted collagen lattices.
Methods Serum-free conditioned medium from melanoma cell lines (C8161, MV3, A375 and Hs294T) was examined for its ability to stimulate the incorporation of 3H-glucosamine and 35SO4 into glycosaminoglycans synthesized by fibroblasts.
Results Conditioned medium from all four melanoma cell lines exhibited potent glycosaminoglycan-stimulating activity. In monolayer culture, C8161-conditioned medium stimulated a 4·2-fold increase in fibroblast hyaluronan, and a 9·9-fold increase in sulphated glycosaminoglycan synthesis, while 35SO4 incorporation was increased only 2·1-fold. In collagen lattice cultures, C8161-conditioned medium stimulated a 4·9-fold increase in hyaluronan synthesis, a 5·4-fold increase in sulphated glycosaminoglycans, and a 1·3-fold increase in 35SO4 incorporation.
Conclusions Melanoma cells produce factors that are potent stimulators of fibroblast glycosaminoglycan synthesis, in both monolayer culture and within contracted collagen lattices. Synthesis of both hyaluronan and sulphated glycosaminoglycans with a reduced degree of polymer sulphation is stimulated. Such changes are likely to promote tumour cell proliferation and migration.