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Inhibition of ultraviolet B radiation-induced interleukin 10 expression in murine keratinocytes by selenium compounds

Authors


Dr Roderick C.McKenzie. E-mail: Roddie.McKenzie@ed.ac.uk

Abstract

SummaryBackground Selenium is an essential trace nutrient necessary for the normal function of the immune system. Selenium compounds protect mice against ultraviolet (UV) B-induced tumours, probably by preventing oxidative damage to the host skin cells and to the host immune system. One possible mechanism of protection is that selenium can prevent oxidative stress-induced release of cytokines such as interleukin (IL)-10, which could suppress cell-mediated immunity.

Objectives To determine whether selenium compounds can inhibit UVB induction of IL-10 protein in murine keratinocytes.

Methods The murine keratinocyte cell line PAM 212 was treated with or without selenomethionine (50–200 nmol L−1) or sodium selenite (1–50 nmol L−1) for 24 h before exposure to 200 J m−2 UVB. The cells were stained with an antibody to IL-10, 24 h after irradiation.

Results Preincubation with both selenium compounds inhibited UVB induction of IL-10 immunostaining, although selenomethionine was more effective. Pretreatment with 200 nmol L−1 selenomethionine decreased IL-10 immunostaining to levels seen in the unirradiated controls.

Conclusions The protective effects of selenium against UVB-induced skin cancer in murine models may result, in part, from its ability to inhibit release of cytokines that are capable of suppressing cell-mediated immunity.

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