Increased frequency of intracellular interleukin (IL)-13 and IL-10, but not IL-4, expressing CD4+ and CD8+ peripheral T cells of patients with atopic dermatitis
Article first published online: 9 DEC 2002
British Journal of Dermatology
Volume 147, Issue 6, pages 1135–1141, December 2002
How to Cite
Aleksza, M., Irinyi, B., Lukács, A., Antal-Szalmás, P., Hunyadi, J. and Szegedi, A. (2002), Increased frequency of intracellular interleukin (IL)-13 and IL-10, but not IL-4, expressing CD4+ and CD8+ peripheral T cells of patients with atopic dermatitis. British Journal of Dermatology, 147: 1135–1141. doi: 10.1046/j.1365-2133.2002.05013.x
- Issue published online: 9 DEC 2002
- Article first published online: 9 DEC 2002
- Accepted for publication 24 April 2002
- atopic dermatitis;
- CD4+ cells;
- CD8+ cells;
- flow cytometry;
Summary Background A number of studies exist demonstrating the increased expression of type 2 cytokines and decreased capacity to produce interferon-γ (IFN-γ) in peripheral blood mononuclear cells (PBMCs) of patients with atopic dermatitis (AD).
Objectives To clarify the results of recent studies concerning the role of interleukin (IL)-4 and IL-13 in PBMCs of AD patients, we analysed the activation status of lymphocyte subpopulations.
Methods We measured the intracellular expression and serum levels of certain type 1 and type 2 cytokines, using cell surface and intracellular cytokine staining, flow cytometry and enzyme-linked immunosorbent assay techniques.
Results The frequency of IL-10 and IL-13 producing CD4+ and CD8+ T cells was significantly higher in patients with AD, while the frequency of IFN-γ secreting helper and cytotoxic T cells was significantly lower in patients with AD than in control subjects. The serum levels of IL-10 and IL-13 were also significantly increased. There were no significant differences observed between the experimental groups in the frequency of IL-4 producing CD4+ and CD8+ cells.
Conclusions This study demonstrates a type 2 cytokine production in the CD4+ and CD8+ T cells of AD patients, which is characterized by an elevated IL-13, but not by IL-4 secretion, and by an increased level of the immunoregulatory IL-10, which can contribute to a decrease in IFN-γ expression.