Deletion of a consensus oestrogen response element half-site in the glucocorticoid receptor of human multiple myeloma

Authors

  • Jayaprakash D. Karkera,

    1. Medical Oncology Section,Department of Veterans Affairs Medical Center, Washington D.C.,
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  • Susan E. Taymans,

    1. Unit of Gene Mapping and Expression, Clinical Neurogenetics Branch, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland, U.S.A.
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  • Gordon Turner,

    1. Unit of Gene Mapping and Expression, Clinical Neurogenetics Branch, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland, U.S.A.
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  • Takeo Yoshikawa,

    1. Unit of Gene Mapping and Expression, Clinical Neurogenetics Branch, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland, U.S.A.
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  • Sevilla D. Detera-Wadleigh,

    1. Unit of Gene Mapping and Expression, Clinical Neurogenetics Branch, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland, U.S.A.
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  • Robert G. Wadleigh

    1. Medical Oncology Section,Department of Veterans Affairs Medical Center, Washington D.C.,
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Dr Robert G. Wadleigh Medical Oncology Section, Department of Veterans Affairs Medical Center, 50 Irving St. NW, Washington DC 20422, U.S.A.

Abstract

We have carried out molecular scanning of the glucocorticoid receptor (GR) of the glucocorticoid resistant multiple myeloma cell line U266. An amplified fragment from the 3′ untranslated region displayed an aberrant migration by PCR–single-stranded conformational polymorphism (PCR-SSCP) analysis. The mutant allele had a deletion of an 8 base pair sequence containing a half-site of an oestrogen response element. This motif was found conserved in rat GR. This same allele lacked four As in an upstream region with 18 consecutive As in the normal allele. These mutations may affect mRNA stability or alter interactions with regulatory factors.

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