Multiple myeloma: reduced plasma cell contamination in peripheral blood progenitor cell collections performed after repeated high-dose chemotherapy courses

Authors

  • Paola Omedè,

    1. Dipartimento di Medicina ed Oncologia Sperimentale, Divisione Universitaria di Ematologia, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy
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  • Corrado Tarella,

    1. Dipartimento di Medicina ed Oncologia Sperimentale, Divisione Universitaria di Ematologia, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy
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  • Antonio Palumbo,

    1. Dipartimento di Medicina ed Oncologia Sperimentale, Divisione Universitaria di Ematologia, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy
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  • Chiara Argentino,

    1. Dipartimento di Medicina ed Oncologia Sperimentale, Divisione Universitaria di Ematologia, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy
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  • Daniele Caracciolo,

    1. Dipartimento di Medicina ed Oncologia Sperimentale, Divisione Universitaria di Ematologia, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy
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  • Paolo Corradini,

    1. Dipartimento di Medicina ed Oncologia Sperimentale, Divisione Universitaria di Ematologia, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy
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  • Alida Dominietto,

    1. Dipartimento di Medicina ed Oncologia Sperimentale, Divisione Universitaria di Ematologia, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy
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  • Fulvia Giaretta,

    1. Dipartimento di Medicina ed Oncologia Sperimentale, Divisione Universitaria di Ematologia, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy
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  • Roberta Ravaglia,

    1. Dipartimento di Medicina ed Oncologia Sperimentale, Divisione Universitaria di Ematologia, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy
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  • Roberta Triolo,

    1. Dipartimento di Medicina ed Oncologia Sperimentale, Divisione Universitaria di Ematologia, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy
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  • Sabrina Triolo,

    1. Dipartimento di Medicina ed Oncologia Sperimentale, Divisione Universitaria di Ematologia, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy
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  • Alessandro Pileri,

    1. Dipartimento di Medicina ed Oncologia Sperimentale, Divisione Universitaria di Ematologia, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy
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  • Mario Boccadoro

    1. Dipartimento di Medicina ed Oncologia Sperimentale, Divisione Universitaria di Ematologia, Azienda Ospedaliera S. Giovanni Battista, Torino, Italy
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Dr Paola OmedèDipartimento di Medicina ed Oncologia Sperimentale, Cattedra di Ematologia, via Genova 3, 10126 Torino, Italy.

Abstract

The possibility of reducing tumour cell contamination by cytotoxic drug courses prior to peripheral blood progenitor cell (PBPC) collection was evaluated in two consecutives groups of multiple myeloma (MM) patient candidates for autograft. All patients were at disease onset and received two VAD (vincristine, doxorubicin and dexamethasone) courses as initial debulking. In the first group (44 patients), mobilization and harvest were performed ‘upfront’, after a single cyclophosphamide (CY) administration of 4 g/m2; in the second group (17 patients), PBPC were collected at the end of a high-dose sequential chemotherapy programme, including: CY 5 g/m2, etoposide (VP16) 2 g/m2, a chemotherapy-free interval with three courses of high-dose dexamethasone, a final mobilizing CY at 7 g/m2. G-CSF was given following each high-dose cytotoxic drug. Cytofluorimetric analysis was performed to quantify progenitors (CD34+ cells) and plasma cells, identified by the high CD38 expression and/or CD38 and CD138 coexpression. Large amounts of PBPC were collected in either group (median harvested CD34+/kg: 15.8 × 106 and 13.4 × 106, respectively; P = 0.9). Circulating plasma cells were significantly higher in patients mobilized ‘upfront’ compared to those who received the high-dose sequence (median peak values of CD38bright/μl: 39 and 10, respectively; P = 0.02); a similar difference was observed in the amount of con-taminating plasma cells in the harvest products (median CD38bright/kg: 7.4 × 106 and 1.3 × 106, respectively; P = 0.02). The results demonstrate that an in vivo purging approach is feasible in myeloma patients through repeated high-dose chemotherapy courses; this may provide less-contaminated material suitable for further in vitro purging procedures.

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