Two M-components in a single cell lineage in a patient with a dual isotype secretory B-cell tumour
Article first published online: 25 DEC 2001
British Journal of Haematology
Volume 102, Issue 3, pages 791–794, August 1998
How to Cite
Sakashita, C., Saito, T., Kurosu, T., Yoshinaga, H., Kumagai, T., Yamamoto, K., Miki, T., Koyama, T., Miura, O., Nemoto, T., Asakawa, H. and Hirosawa, S. (1998), Two M-components in a single cell lineage in a patient with a dual isotype secretory B-cell tumour. British Journal of Haematology, 102: 791–794. doi: 10.1046/j.1365-2141.1998.00832.x
- Issue published online: 25 DEC 2001
- Article first published online: 25 DEC 2001
- biclonal gammopathy;
- multiple myeloma;
- Waldenström's macroglobulinaemia;
- isotypic variants.
We describe a case of Waldenström's macroglobulinaemia with two M-components (IgM and IgG) with the same λ light chain. Southern blot analysis of bone marrow cells showed rearrangements of immunoglobulin heavy and λ light chain genes.
Sequencing of the complementarity determining region 3 of the two γ and μ transcripts showed 100% homology. Immunofluorescence study showed that most cells stained for both IgG and IgM. These findings indicated that a single population of cells was expressing two isotypic variants of IgG and IgM, as the genes responsible for production of both components had the same origin.