• mantle cell lymphoma;
  • large cell variant;
  • PRAD1/ cyclin D1 ;
  • cell line;
  • CD5

A mantle cell lymphoma (MCL) cell line (JeKo-1) was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukaemic conversion. JeKo-1 cells were Epstein-Barr virus negative and showed a B-cell phenotype with IgM+, IgD+, CD3, CD5+, CD10, CD19+, CD20+ and CD23; they overexpressed cyclin Dl, Bcl-2, c-Myc and Rb proteins. Bcl-1/JH gene rearrangement was confirmed by polymerase chain reaction, although karyotypic analysis showed 40/41 chromosomes devoid of apparent t(11;14)(q13;q32) translocation. JeKo-1 cells were highly tumourigenic in SCID mice.