Assessment of thrombocytopenic disorders using the Platelet Function Analyzer (PFA-100®) *


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    Presented in part at the XVIIth Congress of the International Society on Thrombosis and Haemostasis, Washington DC, USA, August 1999.

Dr M. L. Rand, Division of Haematology/Oncology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada. E-mail:


Summary.  The Platelet Function Analyzer (PFA-100®) was used to measure platelet function in paediatric patients with destructive versus underproduction thrombocytopenia. Closure time (CT) and total volume (TV) measurements with standard 150 μm apertures discriminated between patients with similar platelet counts from 30 to 150 × 109/l. However, at platelet counts < 30 × 109/l, a 100-μm aperture (experimental) gave the best assessment of platelet function. TV results could be analysed even when CTs were indeterminate. Further investigations are warranted to more fully understand the relationships among platelet function as measured by the PFA-100® in standard/experimental modes, bleeding and transfusion outcome in thrombocytopenia.