A subset of CD8+ T cells from allergic patients produce IL-4 and stimulate IgE production in vitro
Article first published online: 27 APR 2006
Clinical & Experimental Allergy
Volume 27, Issue 12, pages 1402–1411, December 1997
How to Cite
MEISSNER, N., KUSSEBI, E., JUNG, T., RATTI, H., BAUMGARTEN, C., WEREEL, T., HEUSSER, C. and RENZ, H. (1997), A subset of CD8+ T cells from allergic patients produce IL-4 and stimulate IgE production in vitro. Clinical & Experimental Allergy, 27: 1402–1411. doi: 10.1046/j.1365-2222.1997.1180931.x
- Issue published online: 27 APR 2006
- Article first published online: 27 APR 2006
- Submitted 29 August 1996; revised 25/ February 1997; accepted 25 April 1997.
- IgE production;
- CD8+ T cells;
Background and Objective A subset of IL-4 producing CD8+ T cells was recently identified in HIV patients. Based on these findings we examined whether IL-4 producing CD8+ T cells would also be present in allergic patients and what would be the functional relevance of this T-cell population.
Methods We investigated the role of CD8+ T cells in IgE production of allergic diseases by analysing the cytokine profile of individual CD4+ and CD8+ T cells.
Results In allergic patients about twice as many CD4+ T cells and six times as many CD8+ T cells produced IL-4 as in non-allergic controls. In contrast the frequency of IFNγ+ T-cell subsets did not significantly differ between the allergic and non-allergic individuals. The frequency of 1L4+CD8+ T cells correlated with the level of serum IgE. Coculture experiments with T cells or purified CD8+ T cells together with autologous B cells indicated that CD8+ T cells enhanced IgE in vitro, but not IgM production, even when they were physically separated from B cells. This effect could be partially blocked by addition of an IL-4 binding protein, a soluble IL-4 receptor indicating that lL-4 is involved in CD8+ T-cell mediated IgE production.
Conclusions These data indicate a positive role of IL-4 secreting CD8+ T cells in IgE regulation in allergic patients.