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GC/MS analysis of urinary excretion of 9α,11β-PGF2 in acute and exercise-induced asthma in children

Authors


Dr T. Nagakura, Division of Allergy, Department of Paediatrics, Jikei University School of Medicine, 3-25-8, Nishi-Shinbashi, Minato-ku, Tokyo, 105, Japan.

Abstract

Background

9α,11β-Prostaglandin (PG) F2 is an initial metabolite of PGD2 which has a potent bronchoconstrictive activity.

Objectives

We measured the urinary levels of 9α,11β-PGF2 in asthmatic children to investigate its role in not only acute asthmatic attack in a time course study but also in exercise-induced asthma (EIA).

Methods

In the acute asthma study, 30 asthmatic children were examined. Urine samples were collected on the first, third, and sixth days. Urinary levels of 9α,11β-PGF2 were measured with gas chromatography mass spectrometry using the electron impact method. In the exercise challenge study, 14 children with EIA and 14 children without EIA were studied. Urine samples were collected before exercise challenge, and at 1 h, and 5 h after exercise challenge. Urinary levels of 9α,11β-PGF2 were measured.

Results

Elevated urinary levels of 9α,11β-PGF2, which were observed on the first day when treatment was started in the hospital, were gradually decreased on the third day (P < 0.05), and on the sixth day (P < 0.01). A significant correlation between urinary levels of 9α,11β-PGF2 and symptom scores (P < 0.005) was observed on the first day. In EIA, there was a significant increase in urinary levels of 9α,11β-PGF2 at 1 h (P < 0.01) and at 5 h (P < 0.01) after exercise challenge, but not in the children without EIA.

Conclusion

9α,11β-PGF2 may be involved in the pathogenesis of acute and exercise-induced asthma in children.

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