Olive pollen allergy: searching for immunodominant T-cell epitopes on the Ole e 1 molecule
Article first published online: 25 DEC 2001
Blackwell Science Ltd, Oxford
Clinical & Experimental Allergy
Volume 28, Issue 4, pages 413–422, April 1998
How to Cite
CÁrdaba, Del Pozo, Jurado, Gallardo, Cortegano, Arrieta, Del Amo, TramÓN, Florido, Sastre, Palomino and Lahoz (1998), Olive pollen allergy: searching for immunodominant T-cell epitopes on the Ole e 1 molecule. Clinical & Experimental Allergy, 28: 413–422. doi: 10.1046/j.1365-2222.1998.00190.x
- Issue published online: 25 DEC 2001
- Article first published online: 25 DEC 2001
- Ole e 1;
- HLA class II;
- T-cell epitopes;
- olive pollen sensitization;
- main allergen of olive pollen
The amino-acid and nucleotide sequence of Ole e 1 (the major antigen of olive pollen) has been described and the IgE antibody response to this major allergen was associated with DR7/DQ2 antigens. With this previous data we try to define the T-cell epitopes implicated in Ole e 1 reactivity.
To study the recognition of T cells (derived from allergic and non-allergic Ole e 1 patients) to Ole e 1 synthetic peptides in order to define immunodominant T-cell epitopes.
We have compared the proliferative response of the peripheral blood mononuclear cells from Ole e 1 sensitized patients vs. non-sensitized controls, induced by 14 Ole e 1 synthetic peptides. Thirty subjects were classified in two groups: group 1 (non-responders against Ole e 1, n = 16) and group 2 (Ole e 1 responders, n = 14), according to their clinical parameters and the presence or not in their sera of the significant Ole e 1 IgE antibody levels.
Our results shown that it is possible to find T cells reactive to Ole e 1 peptides in patients with and without significant levels of Ole e 1 IgE antibodies. However, the percentage of response was higher in patients with IgE antibodies 71.4% vs 25%), and the recognition profile was different: the control group showed a broad reactivity pattern, in contrast, the response by the ‘Ole e 1 responders’ group was mainly directed against three peptides of the carboxi-terminal region, peptides 10 (91–102), 12 (109–120) and 13 (119–130), with a response frequency of 35.7, 28.5 and 28.5%, respectively. By direct and inhibition test no antibody response was found against the synthetic peptides.
Our data suggest that the regions between 91 and 102 and 109–130 amino-acids on the Ole e 1 molecule are immunodominant T-cell epitopes. These epitopes are not recognized by IgE antibodies.