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Elevated levels of soluble TNF receptors in bronchoalveolar lavage fluid in extrinsic allergic alveolitis

Authors


Costabel Ruhrlandklinik, Tüschener Weg 40, D-45239 Essen, Germany.

Abstract

Background

Soluble TNF receptors (sTNFR1 and sTNFR2) are inhibitors of TNF and can block TNF bioactivity. TNF plays an important role in the development of extrinsic allergic alveolitis (EAA).

Objective

To evaluate whether sTNFR1 and sTNFR2 are locally increased in EAA.

Methods

We measured sTNFR1 and sTNFR2 in bronchoalveolar lavage fluid (BALF) and serum from nine EAA patients and 11 control subjects using an ELISA method.

Results

BALF sTNFR1 and sTNFR2 levels were 0.24 ± 0.04 ng/mL and 0.59 ± 0.16 ng/mL in EAA patients, and thus significantly elevated in comparison with the controls (0.13 ± 0.02 ng/mL and 0.08 ± 0.04 ng/mL, both P < 0.05). Serum sTNFR levels were not significantly different between the two groups. Both sTNFR1 and sTNFR2 concentrations in BALF correlated significantly with the lymphocyte percentage of BALF (r = 0.57 and 0.81, respectively).

Conclusion

The two alveolar sTNFRs, particularly sTNFR2, may be involved in the pathogenesis of EAA as counter-regulators of TNF.

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