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Keywords:

  • human lung mast cell;
  • interleukin-10;
  • rhSCF

Background

Mast cells control the local inflammation by producing many kinds of cytokines. Interleukin (IL)-10 is one of the important cytokine that upregulate or downregulate inflammation.

Objective

The aim of this study is to ascertain whether IL-10 is produced from human lung mast cells by cross-linkage of high-affinity Fcε receptors (FcεRI).

Methods

Mast cells were purified using affinity magnetic selection with mAb YB5.B8 (> 93% pure). Mast cells were precultured with human myeloma IgE (3 μg/mL) for 16 h and then washed, and stimulated with anti-IgE in the presence or absence of recombinant human stem cell factor (rhSCF). We have studied the production of IL-10 by using reverse transcription-PCR, enzyme-linked immunosorbent assay and immunocytochemistry.

Results

We found that human lung mast cells were immunocytochemically stained with anti-IL-10 mAb after IgE-dependent stimulation. The activation of mast cells via FcεRI enhanced the intensity of the IL-10 mRNA signal. Anti-IgE (1 μg/mL) induced a median IL-10 release of 301.7 (7.8–1532.4) pg/106 mast cells/24 h. In contrast, mast cells released only a small amount of IL-10 in the absence of anti-IgE. This difference was statistically significant (P = 0.02, n = 11).

Conclusion

Our findings indicate that human lung mast cells are capable of producing IL-10 in response to IgE-dependent stimulation.