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Clinical & Experimental Allergy

Influence of bronchial allergen challenge on histamine release by human basophils

Authors

  • Lie,

    1. Central Laboratory of The Netherlands Red Cross Blood Transfusion Service and the Laboratory for Experimental and Clinical and Immunology, Academic Medical Center, University of Amsterdam, Amsterdam,
    2. Department of Pulmonology, Academic Medical Center, Amsterdam,
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  • Van Der Veen,

    1. Department of Pulmonology, Academic Medical Center, Amsterdam,
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  • Knol,

    1. Central Laboratory of The Netherlands Red Cross Blood Transfusion Service and the Laboratory for Experimental and Clinical and Immunology, Academic Medical Center, University of Amsterdam, Amsterdam,
    2. Department of Dermatology/Allergology, University Medical Center Utrecht, Utrecht, The Netherlands
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  • Mul,

    1. Central Laboratory of The Netherlands Red Cross Blood Transfusion Service and the Laboratory for Experimental and Clinical and Immunology, Academic Medical Center, University of Amsterdam, Amsterdam,
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  • Jansen,

    1. Department of Pulmonology, Academic Medical Center, Amsterdam,
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  • Roos,

    1. Central Laboratory of The Netherlands Red Cross Blood Transfusion Service and the Laboratory for Experimental and Clinical and Immunology, Academic Medical Center, University of Amsterdam, Amsterdam,
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  • Van Der Zee

    1. Department of Pulmonology, Academic Medical Center, Amsterdam,
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Roos Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Plesmanlaan 125, 1066 CX Amsterdam, The Netherlands.

Abstract

Background

Basophils can be primed by cytokines such as interleukin (IL) -3, IL-5 or granulocyte macrophage-colony stimulating factor (GM-CSF). It has been described that the concentrations of these cytokines are enhanced at sites of allergic inflammation as well as systemic in allergic asthma.

Objective

To investigate the priming status of basophils as detected by thapsigargin-induced histamine release during bronchial allergen challenge.

Methods

Ten subjects allergic to house dust mite were challenged via an aerosol delivery system. Spontaneous leucocyte histamine release as well as histamine release induced by various stimuli was measured in vitro at several time points. In addition, lung function parameters, serum IL-5 and blood eosinophil counts were evaluated.

Results

We found no effect of bronchial allergen challenge upon spontaneous leucocyte histamine release, nor upon histamine release induced by anti-immunoglobulin (Ig) E, house dust mite extract, C5a, fMLP, IL-3, PMA+ thapsigargin or IL-3+ thapsigargin. However, the priming status of basophils as measured by thapsigargin-induced histamine release was enhanced at 24 h after bronchial allergen challenge. Analysis of the individual data showed a heterogeneous initial response (30 min, 6 h) followed by a predominant increase at 24 h after allergen challenge. This increase in the thapsigargin-induced histamine release correlated with the increase in serum IL-5 levels at 24 h after allergen challenge.

Conclusion

The priming status of human basophils as measured by thapsigargin-induced histamine release is enhanced 24 h after allergen challenge.

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