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Activation of fibroblasts in collagen lattices by mast cell extract: a model of fibrosis

Authors


Prof. François-Xavier Maquart Laboratoire de Biochimie, Faculté de Médecine, 51 rue Cognacq-Jay, 51095 Reims Cedex, France.

Abstract

Background

Mast cells are resident connective tissue cells able to secrete numerous inflammatory mediators in response to tissue aggression and might be implicated in the fibrotic processes.

Objectives

To study the effects of mast cell products on fibroblast activity in connective tissues.

Methods

Mast cell extract was prepared by sonication of pure mast cell preparations obtained by peritoneal lavage of rats and added to the culture medium of fibroblast-populated collagen lattices.

Results

Mast cell extract was able to decrease the contraction of the collagen lattices, to stimulate total protein and collagen synthesis, and to increase the expression and activation of gelatinase A/MMP-2.

Conclusion

These data are consistent with the hypothesis that mast cells in connective tissue may be responsible for fibroblast activation at the early phases of tissue repair and fibrosis.

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