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Macrophage migration inhibitory factor (MIF) in bronchial asthma


Yamaguchi The First Department of Medicine, School of Medicine, Hokkaido University, Kita-15 Nishi-7 Kitaku, Sapporo 060–8638, Japan.



Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine favouring the secretion of TNFα and IL-8 and counteracts anti-inflammatory effects of corticosteroids. Airways inflammation is a central feature of bronchial asthma and is characterized by the accumulation of eosinophils.


The aim of this study was to investigate whether MIF is related to asthma symptoms and eosinophil accumulation in the airways.


Serum MIF levels were measured by an enzyme-linked immunosorbent assay in 44 healthy subjects and 44 asthmatics. Levels of MIF in induced sputum were measured in 10 healthy subjects and 15 asthmatics. Levels of eosinophil cationic protein (ECP) in induced sputum were measured by a radioimmunosorbent assay. Fluorescence double immunostaining was conducted to examine cellular source and localization of MIF.


Serum MIF levels were significantly increased in asthmatic patients compared with age and sex-matched control subjects. Symptomatic patients had a higher MIF level than asymptomatic patients. Induced sputum obtained from asthmatics contained higher levels of MIF than those from control subjects. MIF levels in induced sputum were correlated with ECP levels in induced sputum. MIF was colocalized with eosinophil peroxidase staining in the cytoplasm of sputum cells.


Increased MIF levels are associated with asthma symptoms and one of the cellular sources of MIF in the airways are eosinophils.