IgE receptor-mediated release of nerve growth factor by mast cells
Article first published online: 5 APR 2002
Clinical & Experimental Allergy
Volume 30, Issue 10, pages 1379–1386, October 2000
How to Cite
Xiang, Z. and Nilsson, G. (2000), IgE receptor-mediated release of nerve growth factor by mast cells. Clinical & Experimental Allergy, 30: 1379–1386. doi: 10.1046/j.1365-2222.2000.00906.x
- Issue published online: 5 APR 2002
- Article first published online: 5 APR 2002
- mast cells;
- nerve growth factor
It has previously been demonstrated that the level of nerve growth factor (NGF) is increased in serum from humans with allergic diseases and asthma.
A question raised by these observations is whether NGF could be released from degranulating mast cells during an allergic reaction. The aim of this study was to investigate if NGF is released from mast cells after activation through cross-linkage of the high-affinity IgE receptor.
Mouse and human in vitro cultured mast cells were activated by IgE and specific antigen, stem cell factor or lipopolysaccharide. Release of NGF was measured by ELISA and mRNA expression was detected by RT PCR.
We found that mast cells not only express NGF transcripts, but also release NGF polypeptide in response to IgE and specific antigen. Activation of mouse mast cells for 30 min induced significant release of NGF (32.9 ± 1.3 pg/2 × 106 cells) compared to spontaneous release (13.9 ± 2.7 pg/2 × 106 cells) (P < 0.01). Similarly, activation of human cultured mast cells also resulted in a significant increase of NGF release (733 ± 310 pg/3 × 105 cells) compared to spontaneous release (9.2 ± 4.0 pg/3 × 105 cells). Two other mast cell secretogogues studied, stem cell factor and lipopolysaccharide, were not able to induce release of NGF.
This study provides evidence that NGF could be specifically released by stimuli causing an allergic reaction, and mast cells can thereby be the source of NGF in IgE-mediated allergic diseases. Our findings add further support for a close correlation between NGF and mast cells that could be of importance for the allergic inflammation.