Rapid polarization of Th2 cells during induction of antigen-specific IgE antibodies in vitro
Article first published online: 24 DEC 2001
Clinical & Experimental Allergy
Volume 30, Issue 9, pages 1298–1306, September 2000
How to Cite
Åkesson, Ingvarsson, Brady, Moynagh and Borrebaeck (2000), Rapid polarization of Th2 cells during induction of antigen-specific IgE antibodies in vitro. Clinical & Experimental Allergy, 30: 1298–1306. doi: 10.1046/j.1365-2222.2000.00912.x
- Issue published online: 24 DEC 2001
- Article first published online: 24 DEC 2001
- Antigen specific;
- in vitro;
- immune deviation
Type 2 T-helper cells (Th2) are involved in the regulation of the humoral immune response against antigens and allergens and directly affect which isotype will be produced. The mechanism that regulates antigen-specific IgE secretion and immune deviation is still not known.
To delineate mechanisms behind antigen-specific IgE secretion we have used in vitro immunization and focused on T-cell phenotype and the activation status of the transcription factor NFκB.
Peripheral blood lymphocytes (PBMC) from seronegative donors were immunized in vitro with a peptide consisting of both a T-cell and a B-cell epitope.
Antigen-specific IgE antibodies could be detected after a primary immunization, during which T-helper cells secreted type 2 cytokines. Specific IgE was also detected in the secondary immunization, but due to a rapid polarization from Th2 to Th1 phenotype, exogenous IL-4 was required for the specific IgE secretion. Analysis of NFκB activation in B and T cells during primary and secondary immunization showed that NFκB could be detected in both B and T cells during primary immunization, but was dependent on exogenous IL-4 in the secondary immunization.
This is the first evidence of antigen-specific IgE induction in vitro using naive B cells, demonstrating the involvement of T-helper cell phenotype and NFκB and demonstrates the usefulness of in vitro cultures to study the effect of antigens on human immunocytes.