Microflora-associated characteristics in faeces from allergic and nonallergic infants
Article first published online: 5 SEP 2008
Blackwell Science Ltd
Clinical & Experimental Allergy
Volume 30, Issue 11, pages 1591–1596, November 2000
How to Cite
Böttcher, M. F., Nordin, E. K., Sandin, A., Midtvedt, T. and Björkstén, B. (2000), Microflora-associated characteristics in faeces from allergic and nonallergic infants. Clinical & Experimental Allergy, 30: 1591–1596. doi: 10.1046/j.1365-2222.2000.00982.x
- Issue published online: 5 SEP 2008
- Article first published online: 5 SEP 2008
- Submitted 19 July 1999; revised 18 November 1999; accepted 24 February 2000.
- microflora-associated characteristic;
- intestinal microflora;
- short chain fatty acids
The prevalence of allergic diseases has increased particularly over the past 30–40 years. A reduced microbial stimulation during infancy may result in a development of a disturbed balance between Th1- and Th2-like immunity. The gut flora is, quantitatively, the most important source for such stimulation.
The aim of the study was to compare the gut microbial flora in 25 allergic and 47 nonallergic 13-month-old infants (range 11–18), through analysing microflora-associated biochemical markers in faeces.
Microflora associated characteristics (MACs) were assessed by determining the concentrations of eight different short chain fatty acids and the conversion of cholesterol to coprostanol by gas chromatography. Faecal tryptic activity was analysed spectrophotometrically.
The allergic infants had lower levels of propionic, i-butyric, butyric, i-valeric and valeric acid. In contrast, they had higher levels of the rarely detected i-caproic acid, which has been associated with the presence of Clostridium difficile. Furthermore, the allergic infants had higher relative distribution of acetic and i-caproic acid. None of the other parameters differed between the groups.
The results demonstrate differences in the MACs between allergic and nonallergic infants, indicating differences in the composition of the gut flora that may disturb the development of a normal Th1-/Th2-balance in allergic children.