Allergen-induced bronchial inflammation is associated with decreased levels of surfactant proteins A and D in a murine model of asthma
Article first published online: 12 JAN 2002
Clinical & Experimental Allergy
Volume 31, Issue 4, pages 652–662, April 2001
How to Cite
Wang, J. Y., Shieh, C. C., Yu, C. K. and Lei, H. Y. (2001), Allergen-induced bronchial inflammation is associated with decreased levels of surfactant proteins A and D in a murine model of asthma. Clinical & Experimental Allergy, 31: 652–662. doi: 10.1046/j.1365-2222.2001.01031.x
- Issue published online: 12 JAN 2002
- Article first published online: 12 JAN 2002
- Submitted 21 March 2000; revised 28 July 2000; accepted 28 August 2000.
- airway allergic inflammation;
- surfactant proteins;
- bronchial asthma
Background Increasing evidence suggests that pulmonary surfactant protein A (SP-A) and D (SP-D) participate in the lung defence against pathogens. However, the role of surfactant proteins in the pathogenesis of allergen-induced airway inflammation has not been elucidated. In this study we examined the levels and distributions of SP-A and SP-D in a dust mite (Dermatophagoides pteronyssinus, Der p) allergen-induced murine model of asthma.
Methods The concentration of SP-A and SP-D in the bronchoalveolar lavage fluid (BALF) and the distribution of surfactant proteins in the lung were assayed by ELISA and immunohistochemistry methods, respectively. The effect of surfactant proteins on allergen-induced pulmonary lymphocyte proliferation was also studied.
Results We demonstrated that there were marked reductions of SP-A and SP-D levels in the BALF of Der p-sensitized BALB/c mice at 48–72 h after allergen challenge (AC). Both purified SP-A and SP-D were able to suppress, in a dose dependent manner, Der p-stimulated intrapulmonary lymphocyte proliferation of naïve mice with saline or allergen challenge, or of Der p-sensitized mice with saline challenge. On the contrary, this suppressive effect was mild (< 9%) on lymphocytes from sensitized mice after AC.
Conclusion These results indicated the involvement of pulmonary surfactant proteins in the allergic bronchial inflammation of sensitized mice.