Measurement of personal exposure to aerosols of Nephrops norvegicus (Scampi) using a monoclonal-based assay
Article first published online: 12 JAN 2002
Clinical & Experimental Allergy
Volume 31, Issue 6, pages 928–933, June 2001
How to Cite
Griffin, P., Allan, L., Gibson, M., Elms, J., Wiley, K. and Curran, A. D. (2001), Measurement of personal exposure to aerosols of Nephrops norvegicus (Scampi) using a monoclonal-based assay. Clinical & Experimental Allergy, 31: 928–933. doi: 10.1046/j.1365-2222.2001.01100.x
- Issue published online: 12 JAN 2002
- Article first published online: 12 JAN 2002
- Submitted 19 May 2000; revised 13 October 2000; accepted 23 November 2000.
- airborne allergen;
- Nephrops norvegicus;
- air sampling
Background The industrial processing of scampi (Nephrops norvegicus) generates bioaerosols which can cause occupational respiratory disease in exposed individuals. The objective of this study was to develop a monoclonal antibody-based immunoassay to measure scampi proteins collected using standard personal air sampling methods.
Methods A monoclonal antibody was generated which predominantly bound to a single 97 Kd scampi protein, which we have shown to be a major allergen. This protein was shown by SDS PAGE immunoblotting to be present in various aqueous extracts from wastes produced during scampi processing. A quantitative immunoslotblot method was developed to quantify scampi from air sample filters.
Results We demonstrated the utility of this method by showing a significant difference (P = 0.015) in the level of exposure of two groups of 12 workers processing scampi at a traditional open workstation (GM = 607 ng m−3) and at an enclosed workstation (GM = 451 ng m−3).
Conclusion We conclude that this assay can be used, in conjunction with health surveillance, to monitor exposure and the efficacy of measures to reduce exposure to airborne scampi allergen in an occupational environment.