Ambient ozone exposure is associated with eosinophil activation in healthy children
Article first published online: 12 JAN 2002
Clinical & Experimental Allergy
Volume 31, Issue 8, pages 1213–1219, August 2001
How to Cite
Frischer, T., Studnicka, M., Halmerbauer, G., Horak Jr, F., Gartner, C., Tauber, E. and Koller, D. Y. (2001), Ambient ozone exposure is associated with eosinophil activation in healthy children. Clinical & Experimental Allergy, 31: 1213–1219. doi: 10.1046/j.1365-2222.2001.01155.x
- Issue published online: 12 JAN 2002
- Article first published online: 12 JAN 2002
- Submitted 25 February 2000; revised 13 October 2000; accepted 7 February 2001.
Background Eosinophil activation is characteristic for allergic airways disease. However, eosinophilic airways inflammation has also been observed subsequent to ambient ozone exposure.
Methods For a population sample of 877 children living at nine sites with different ozone exposure we measured urinary eosinophil protein X (U-EPX) as a marker of eosinophil activation. U-EPX was determined from a single spot urine sample during autumn 1997. Children were participants in a longitudinal study of ozone effects on lung function.
Results The 5–95% percentiles of ozone exposure (30-day mean before test) were 11.8–51.5 p.p.b. (mean: 31.6 ppb). U-EPX was measured by radioimmunoassay and expressed as ratio to urinary creatinine (µg EPX/mmol creatinine). Log transformation was performed to achieve a normal distribution. LogU-EPX was associated with gender, a diagnosis of asthma and atopy (skin test sensitivity to any of seven aeroallergens). LogU-EPX increased with ozone exposure for all children. The medians of LogU-EPX according to the first–fourth quartiles of ozone exposure were: 1.82, 1.88, 1.95 and 2.03. For 172 non-asthmatic children who had spent the whole summer at their site corresponding figures were 1.57, 1.78, 2.07 and 2.13. In a multivariate model with logU-EPX being the dependent variable and adjusted for gender, site and atopy, ozone was found to be significant (estimate: 0.007 µg/mmol creatinine per ppb ozone; SE:0.02; P < 0.001).
Conclusion Our observation supports the hypothesis that ozone in healthy children is associated with eosinophil inflammation, most likely in the airways.