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Clinical & Experimental Allergy

Effect of lysed Enterococcus faecalis FK-23 (LFK) on allergen-induced peritoneal accumulation of eosinophils in mice

Authors

  • T. Shimada,

    1. Central Research Laboratories, Nichinichi Pharmaceutical Co. Ltd, Mie, Japan,
    2. International Research Centre for Nasal Allergy, Nanjing Medical University and Department of Otorhinolaryngology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China,
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  • L. Cheng,

    1. International Research Centre for Nasal Allergy, Nanjing Medical University and Department of Otorhinolaryngology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China,
    2. Department of Health Promotion and Human Behaviour, Kyoto University Graduate School of Public Health, Kyoto, Japan and
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  • M. Ide,

    1. Central Research Laboratories, Nichinichi Pharmaceutical Co. Ltd, Mie, Japan,
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  • S. Fukuda,

    1. Department of Health Promotion and Human Behaviour, Kyoto University Graduate School of Public Health, Kyoto, Japan and
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  • T. Enomoto,

    1. Department of Otolaryngology, Japanese Red Cross Society Wakayama Medical Center, Wakayama, Japan
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  • T. Shirakawa

    1. Department of Health Promotion and Human Behaviour, Kyoto University Graduate School of Public Health, Kyoto, Japan and
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Takashi Shimada, Central Research Laboratories, Nichinichi Pharmaceutical Co. Ltd, 239–1 Tominaga Oyamada, Ayama-gun, Mie 518–1417, Japan. E-mail: labo@nichinichi-phar.co.jp

Summary

Background The interest in anti-allergy immunoregulation by lactic acid bacteria has been growing for the last few decades. There is some evidence to suggest that lysed Enterococcus faecalis FK-23 (LFK) could relieve the clinical symptoms of pollinosis. However, the mechanism responsible for this phenomenon remains unknown.

Objective To identify the effect of LFK, a lysozyme treated and heat-killed preparation from the lactic acid bacteria Enterococcus faecalis FK-23 strain, on allergen-induced eosinophil accumulation.

Methods BALB/c mice were sensitized with ragweed pollen extract, and peritoneal accumulation of eosinophils was induced. A total of 60 mg (0.5 mL) LFK was orally administered to the experimental mouse every day during 21 days of the sensitization period. In addition, LFK 4 mg, 25 mg and 60 mg (each 0.5 mL) were also orally administered to a mouse of each group every day for 21 days. Saline was fed in a dose of 0.5 mL/mouse per day for the same duration as a control.

Results Compared with control mice, LFK-treated mice exhibited decreased ragweed pollen allergen-induced peritoneal accumulation of eosinophils (P = 0.013), which showed a tendency to be in a dose-dependent fashion (P = 0.14).

Conclusion The results provide laboratory evidence of the role for LFK, a lactic acid bacteria preparation, in combating eosinophil accumulation.

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