Allergen-mediated modulation of CD23 expression is interferon-γ and interleukin-10 dependent in allergic and non-allergic individuals
Article first published online: 7 NOV 2003
Clinical & Experimental Allergy
Volume 33, Issue 11, pages 1568–1575, November 2003
How to Cite
Roever, A. C., Heine, G., Zuberbier, T. and Worm, M. (2003), Allergen-mediated modulation of CD23 expression is interferon-γ and interleukin-10 dependent in allergic and non-allergic individuals. Clinical & Experimental Allergy, 33: 1568–1575. doi: 10.1046/j.1365-2222.2003.01797.x
- Issue published online: 7 NOV 2003
- Article first published online: 7 NOV 2003
- Submitted 17 September 2002; revised 13 April 2003; accepted 13 July 2003
- birch pollen allergen;
- CD23 expression;
Background CD23 plays an important role in IgE regulation. The modulation of CD23 expression during specific immunotherapy (SIT) has been described previously. In the present study, we investigated in detail the effects of complete birch pollen allergen extract (BPA) on CD23 expression of peripheral blood mononuclear cells (PBMCs) in vitro.
Methods PBMCs from 14 birch pollen-allergic (bp-allergic) patients and eight non-bp-allergic controls were stimulated with IL-4 and increasing doses of BPA. CD23 expression on monocytes and B cells was measured by flow cytometry; sCD23 release and the levels of IFN-γ and IL-10 secretion were determined by ELISA. To analyse the mechanisms on CD23 expression in more detail, neutralizing anti-IFN-γ and anti-IL-10 antibodies were added to IL-4 and BPA-stimulated cultures.
Results IL-4 induced CD23 expression on B cells and on monocytes and sCD23 release in the bp-allergic and non-bp-allergic groups. The addition of BPA to IL-4-stimulated PBMC decreased CD23 expression significantly and dose-dependently on B cells in both groups. CD23 expression on monocytes was also decreased in both groups after the addition of BPA, but higher doses were required in the non-bp-allergic population. IL-4-induced sCD23 release was also significantly decreased after the addition of BPA. IFN-γ and IL-10 were induced by BPA in both the bp-allergic and non-bp-allergic groups. The addition of neutralizing anti-IFN-γ antibodies increased CD23 expression on B cells, which were stimulated with IL-4 and BPA, but had no effect on monocytes, whereas the addition of anti-IL-10 antibodies increased CD23 expression on monocytes but not on B cells.
Conclusion These data indicate that early immunological effects like down-regulation of CD23 on B cells and monocytes, which are observed during SIT are dose dependent, mediated by IFN-γ and IL-10 and seem not to depend per se on the sensitization state of an individual.