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IL-10 enhances IL-2-induced proliferation and cytotoxicity by human intestinal lymphocytes

  1. E. C. Ebert

Article first published online: 24 DEC 2001

DOI: 10.1046/j.1365-2249.2000.01147.x

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Clinical & Experimental Immunology

Clinical & Experimental Immunology

Volume 119, Issue 3, pages 426–432, March 2000

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Ebert, E. C. (2000), IL-10 enhances IL-2-induced proliferation and cytotoxicity by human intestinal lymphocytes. Clinical & Experimental Immunology, 119: 426–432. doi: 10.1046/j.1365-2249.2000.01147.x

Author Information

  1. Department of Medicine, UMDNJ Robert Wood Johnson Medical School, New Brunswick, NJ, USA

* EllenC. Ebert Md Department of Medicine, UMDNJ Robert Wood Johnson Medical School, One Robert Wood Johnson Place, New Brunswick, NJ 08903, USA.

Publication History

  1. Issue published online: 24 DEC 2001
  2. Article first published online: 24 DEC 2001

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Keywords:

  • lymphokine-activated killer activity;
  • tumour necrosis factor;
  • interferon-gamma;
  • IL-2;
  • IL-10;
  • inflammatory bowel disease

IL-10 modulation of human intestinal T lymphocyte functions was studied for the first time. Lymphocyte proliferation was determined by 3H-thymidine incorporation; cytokine production, by ELISA; expression of surface markers, by immunofluorescence and flow cytometric analysis; and cytotoxicity, by lysis of 51Cr-labelled target cells. IL-10 blocked phytohaemagglutinin (PHA)-induced activation and proliferation of CD8+ T cells from the epithelium and lamina propria. It was a greater inhibitor of IL-2, interferon-gamma, and tumour necrosis factor-alpha production than were IL-4 or transforming growth factor-beta. In contrast, IL-10 enhanced IL-2-stimulated proliferation of both CD4+ and CD8+ T cells by increasing cell division after activation. It also augmented IL-2- but not IL-15-induced cytotoxicity of intestinal lymphocytes against colon cancer by a mechanism independent of natural killer cells. In conclusion, IL-10 blocking of proinflammatory cytokine secretion probably reduces intestinal inflammation. IL-10 augmentation of IL-2-induced cytotoxicity may help to maintain host defence.

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