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Transfection of single-stranded hepatitis A virus RNA activates MHC class I pathway

  1. K. SUZUKI1,2,
  2. M. YANAGI3,
  3. A. MORI-AOKI1,4,
  4. E. MORIYAMA1,
  5. K. J. ISHII5,
  6. L. D. KOHN1,6

Article first published online: 5 MAR 2002

DOI: 10.1046/j.1365-2249.2002.01767.x

Issue

Clinical & Experimental Immunology

Clinical & Experimental Immunology

Volume 127, Issue 2, pages 234–242, February 2002

Additional Information

How to Cite

SUZUKI, K., YANAGI, M., MORI-AOKI, A., MORIYAMA, E., ISHII, K. J. and KOHN, L. D. (2002), Transfection of single-stranded hepatitis A virus RNA activates MHC class I pathway. Clinical & Experimental Immunology, 127: 234–242. doi: 10.1046/j.1365-2249.2002.01767.x

Author Information

  1. 1

    Cell Regulation Section, Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Maryland, USA,

  2. 2

    Department of Microbiology, Leprosy Research Center, National Institute of Infectious Diseases, Tokyo, Japan,

  3. 3

    Department of Internal Medicine, Kanazawa University School of Medicine, Ishikawa, Japan,

  4. 4

    Department of Internal Medicine, Tottori University School of Medicine, Tottori, Japan,

  5. 5

    Section of Retroviral Immunology, Center for Biologics, Evaluation, and Research, Food and Drug Administration, Maryland, USA, and

  6. 6

    Edison Biotechnology Institute, Ohio University College of Medicine, Athens, OH, USA

* Koichi Suzuki Department of Microbiology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aoba-cho, Higashimurayama, Tokyo 189–0002, Japan. E-mail: koichis@nih.go.jp

Publication History

  1. Issue published online: 5 MAR 2002
  2. Article first published online: 5 MAR 2002

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Keywords:

  • infectious immunity-virus;
  • MHC;
  • antigen presentation;
  • protein kinases/phosphatases

SUMMARY

Although infection of single-stranded RNA viruses can enhance expression of major histocompatibility complex (MHC) class I genes, the mechanism underlying this process remains unclear. Recent studies have indicated that exposure of non-immune cells to double-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) of viral origin can directly increase the expression of MHC class I and related molecules without immune cell interaction. In this report, we show that transfection of single-stranded hepatitis A virus RNA into cultured hepatocytes results in the induction of genes for MHC class I, LMP2 and transporter for antigen processing (TAP1), in addition to the generation of viral proteins. We suggest that this stimulatory effect is due to the double-stranded RNA formed during replication of single-stranded viral RNA, and involves both double-stranded, RNA-dependent protein kinase PKR and the secretion of IFNβ.

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