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Keywords:

  • dendritic cell;
  • LPS;
  • maturation;
  • neonatal;
  • Th1/Th2

SUMMARY

Dendritic cells (DCs) were derived from human peripheral blood monocytes or cord blood monocytes cultured in the presence of IL-4 and GM-CSF. Adult and cord DCs were observed to have comparable immature phenotypes. However, the increase in surface expression of HLA-DR and CD86 after addition of LPS was significantly attenuated in cord DCs, with CD25 and CD83 expression also markedly reduced. Cord DCs were also unable to produce IL-12p70, failed to down-regulate expression of the chemokine receptor CCR5 and induced lower levels of IFN-γ production from allogeneic naive CD4+ T cells than their adult counterparts. In contrast, the kinetics of the production of TNF-α and IL-10 in response to LPS stimulation was comparable to adult DCs. The reduced ability of cord DCs to attain a fully mature adult phenotype, and to activate naive CD4+ T cells to produce IFN-γ, suggests that they are intrinsically preprogrammed against the generation of Th-1 immune responses.