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Memory B lymphocytes determine repertoire oligoclonality early after haematopoietic stem cell transplantation

  1. B. OMAZIC1,*,
  2. I. LUNDKVIST1,
  3. J. MATTSSON2,3,
  4. J. PERMERT1,
  5. I. NÄSMAN-BJÖRK4

Article first published online: 8 SEP 2003

DOI: 10.1046/j.1365-2249.2003.02260.x

Issue

Clinical & Experimental Immunology

Clinical & Experimental Immunology

Volume 134, Issue 1, pages 159–166, October 2003

Additional Information

How to Cite

OMAZIC, B., LUNDKVIST, I., MATTSSON, J., PERMERT, J. and NÄSMAN-BJÖRK, I. (2003), Memory B lymphocytes determine repertoire oligoclonality early after haematopoietic stem cell transplantation. Clinical & Experimental Immunology, 134: 159–166. doi: 10.1046/j.1365-2249.2003.02260.x

Author Information

  1. 1

    Arvid Wretlind Laboratory at the Center for Surgical Science

  2. 2

    Center for Allogeneic Stemcell Transplantation

  3. 3

    Division of Clinical Immunology at the Department of Microbiology, Pathology and Immunology,Karolinska Institutet, Huddinge University Hospital, Sweden and

  4. 4

    Biovitrum, Stockholm, Sweden

*B. Omazic, Arvid Wretlind Laboratory, Center for Surgical Science, KFC, Karolinska Institutet, Huddinge University Hospital, S-141 86 Stockholm, Sweden. E-mail: brigitta.omazic@cfss.ki.se

Publication History

  1. Issue published online: 8 SEP 2003
  2. Article first published online: 8 SEP 2003
  3. (Accepted for publication 22 July 2003)

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Keywords:

  • B lymphocyte repertoire;
  • haematopoietic stem cell transplantation;
  • IgH gene expression;
  • immune reconstitution

SUMMARY

The objective of this study was to investigate if oligoclonality of the Ig repertoire post-haematopoietic stem cell transplantation (HSCT) is restricted to memory B lymphocytes or if it is a general property among B lymphocytes. As a measure of B lymphocyte repertoire diversity, we have analysed size distribution of polymerase chain reaction (PCR) amplified Ig H complementarity determining region 3 (CDR3) in naive and memory B lymphocytes isolated from patients before HSCT and at 3, 6 and 12 months after HSCT as well as from healthy controls. We demonstrate a limited variation of the IgH CDR3 repertoire in the memory B lymphocyte population compared to the naive B cell population. This difference was significant at 3 and 6 months post-HSCT. Compared to healthy controls there is a significant restriction of the memory B lymphocyte repertoire at 3 months after HSCT, but not of the naive B lymphocyte repertoire. Twelve months after HSCT, the IgH CDR3 repertoire in both memory and naive B lymphocytes are as diverse as in healthy controls. Thus, our findings suggest a role for memory B cells in the restriction of the oligoclonal B cell repertoire observed early after HSCT, which may be of importance when considering reimmunization of transplanted patients.

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