Effect of ozone on neutrophil function in vitro

Authors

  • Maya Margalit,

    1. Gaucher Clinic and the Departments of Medicine and Surgery A, Shaare-Zedek Medical Center and Department of Haematology, Hadassah University Hospital (Mount Scopus), Jerusalem, Israel
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  • Eyal Attias,

    1. Gaucher Clinic and the Departments of Medicine and Surgery A, Shaare-Zedek Medical Center and Department of Haematology, Hadassah University Hospital (Mount Scopus), Jerusalem, Israel
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  • Drorit Attias,

    1. Gaucher Clinic and the Departments of Medicine and Surgery A, Shaare-Zedek Medical Center and Department of Haematology, Hadassah University Hospital (Mount Scopus), Jerusalem, Israel
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  • Deborah Elstein,

    1. Gaucher Clinic and the Departments of Medicine and Surgery A, Shaare-Zedek Medical Center and Department of Haematology, Hadassah University Hospital (Mount Scopus), Jerusalem, Israel
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  • Ari Zimran,

    1. Gaucher Clinic and the Departments of Medicine and Surgery A, Shaare-Zedek Medical Center and Department of Haematology, Hadassah University Hospital (Mount Scopus), Jerusalem, Israel
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  • Yaacov Matzner

    1. Gaucher Clinic and the Departments of Medicine and Surgery A, Shaare-Zedek Medical Center and Department of Haematology, Hadassah University Hospital (Mount Scopus), Jerusalem, Israel
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Ari Zimran, Gaucher Clinic, Shaare-Zedek Medical Center, PO Box 3235, Jerusalem 91031, Israel. Tel.: 972 2 655 5143/5673, Fax: 972 2 651 7979.

Abstract

The application of ozone is widely practised as a form of alternative medicine, particularly in Germany and Eastern Europe. Ozone major autohemotherapy (the return of a small amount of a patient’s blood to the circulation after ex vivo exposure to ozone) has been reported to have a therapeutic effect in various pathological conditions, including ischemic, infectious, autoimmune and neoplastic disorders. Ozone has an effect on the expression of cytokines, adhesion molecules and acute phase reactants, which are responsible in part for the respiratory inflammatory response observed after exposure to this gas. The purpose of the present study was to investigate the effect of ozone administration ex vivo, at a concentration commonly used in major autohemotherapy, on peripheral blood neutrophil function in vitro. Blood drawn from healthy volunteers was studied for neutrophil adhesion, chemotaxis and O2 production before and after exposure to 30 μg/ml ozone. There was no significant difference in adhesion and chemotaxis of neutrophils exposed to ozone versus unexposed cells. O2 production was minimally decreased (20.3 ± 5.0 vs. 22.1 ± 5.5 nmol/106 cells/10 min, respectively; P=0.01), a reduction of no clinical significance. This study confirms that major autohemotherapy with ozone is safe as far as neutrophil function is concerned. Combined with previous data, it seems that well-designed clinical trials to assess the efficacy of major autohemotherapy would pose no danger to blood cell populations, and should be encouraged.

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