Cattle MHC genes DOA and DOB: sequence polymorphisms and assignments to the class IIb region

Authors

  • A. Gelhaus,

    Corresponding author
    1. Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Str. 74, 20359 Hamburg, Germany.
      Dr Annette Gelhaus, Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Str. 74, 20359 Hamburg, Germany. Tel.: + 49 40 42818 534; Fax: + 49 40 42818 512; e-mail:gelhaus@bni.uni-hamburg.de
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  • B. Förster

    1. Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Str. 74, 20359 Hamburg, Germany.
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Dr Annette Gelhaus, Department of Molecular Medicine, Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Str. 74, 20359 Hamburg, Germany. Tel.: + 49 40 42818 534; Fax: + 49 40 42818 512; e-mail:gelhaus@bni.uni-hamburg.de

Summary

In a study of the genetic polymorphism of the second exons of the cattle DOA and DOB genes, two and four allelic variants were detected, respectively. In the predicted amino acid sequence, the DOA polymorphism corresponded to variation at the respective residue position, whereas the nucleotide substitutions in the DOB gene were non-informative. PCR-RFLP assays were developed for DOA and DOB typing, and both loci were genetically mapped to the BoLA class IIb region by linkage analysis in the International Bovine Reference Panel. The single nucleotide polymorphisms detected in the BoLA-DOA and -DOB genes enable these loci to be used as markers in genetic trait analyses.

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