Immunohistochemical evidence for mesothelial origin of paratesticular adenomatoid tumour
Article first published online: 25 DEC 2001
Volume 36, Issue 2, pages 109–115, February 2000
How to Cite
Delahunt, Eble, King, Bethwaite, Nacey and Thornton (2000), Immunohistochemical evidence for mesothelial origin of paratesticular adenomatoid tumour. Histopathology, 36: 109–115. doi: 10.1046/j.1365-2559.2000.00825.x
- Issue published online: 12 APR 2007
- Article first published online: 25 DEC 2001
- adenomatoid tumour;
- mesothelial cell;
To investigate the histogenesis of paratesticular adenomatoid tumour by use of immunohistochemical markers for a variety of carcinomas and mesothelioma.
Methods and results
Immunohistochemical staining of sections from 12 cases of paratesticular adenomatoid tumour was undertaken using primary antibodies to antigens expressed by benign epithelial cells and carcinoma (cytokeratin AE1/AE3, cytokeratin 34ßE12, epithelial membrane antigen, MOC-31, Ber-EP4, CEA, B72.3, LEA.135, Leu M1), stromal and vascular markers (vimentin, CD34, factor VIII), and mesothelioma-associated antigens (thrombomodulin, HBME-1, OC 125) and p53 protein. There was absence of immunohistochemical expression of epithelial/carcinoma markers MOC-31, Ber-EP4, CEA, B72.3, LEA.135, Leu M1 and to factor VIII and CD34. All tumours expressed cytokeratin AE1/AE3, epithelial membrane antigen and vimentin, with weak expression of cytokeratin 34ßE12 in 25% of tumours. Each tumour showed expression of thrombomodulin, HBME-1 and OC 125 in a membranous distribution. p53 protein expression was not detected.
The immunohistochemical profile of paratesticular adenomatoid tumour is strongly supportive of a mesothelial cell origin.