Immuno-stimulatory effects of bacterial-derived plasmids depend on the nature of the antigen in intramuscular DNA inoculations

Authors

  • S. W. LEE,

    1. Department of Life Science, Center for Biofunctional Molecules, School of Environmental Engineering, Pohang University of Science and Technology, Hyoja Dong, Pohang, Korea
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  • Y. C. SUNG

    1. Department of Life Science, Center for Biofunctional Molecules, School of Environmental Engineering, Pohang University of Science and Technology, Hyoja Dong, Pohang, Korea
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Dr Y. C. Sung Department of Life Science, Pohang University of Science and Technology, San 31, Hyoja Dong, Pohang, 790-784, Korea.

Abstract

The CpG motifs of bacterial-derived plasmids augment antigen-specific immune responses and steer those responses towards the T helper 1 (Th1) type. In this study, we have addressed the immuno-stimulatory effect of intramuscular co-administration of CpG motifs containing vector DNA on the modulation of immune responses to the haemagglutinin (HA) and the nucleoprotein (NP) proteins of influenza virus. The co-administration of vector DNA with a HA-encoding plasmid DNA showed a significant enhancement in the total IgG response, the generation of cytotoxic T lymphocyte (CTL), and the T-cell proliferative response. In the case of NP-encoding plasmid DNA inoculations, the co-administration of vector DNA slightly decreased the total IgG response, although the IgG2a/IgG1 ratio and the CTL responses to NP were significantly increased. These observations suggest that the immuno-stimulatory effects of bacterial-derived plasmids depend upon the nature of the co-administered antigen.

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