Molecular cloning and characterization of a metal responsive Aedes aegypti intestinal mucin cDNA

  1. A. Rayms-Keller*,
  2. M. McGaw*,
  3. C. Oray*,
  4. J. O. Carlson*,
  5. B. J. Beaty*

Article first published online: 25 DEC 2001

DOI: 10.1046/j.1365-2583.2000.00202.x

Issue

Insect Molecular Biology

Insect Molecular Biology

Volume 9, Issue 4, pages 419–426, August 2000

Additional Information

How to Cite

Rayms-Keller, A., McGaw, M., Oray, C., Carlson, J. O. and Beaty, B. J. (2000), Molecular cloning and characterization of a metal responsive Aedes aegypti intestinal mucin cDNA. Insect Molecular Biology, 9: 419–426. doi: 10.1046/j.1365-2583.2000.00202.x

Author Information

  1. Arthropod-borne & Infectious Disease Laboratory, Center for Environmental Toxicology & Technology, Department of Microbiology, Colorado State University, Fort Collins, Colorado, USA

* *Correspondence author. E-mail: arayms@lamar.colostate.edu

Publication History

  1. Issue published online: 25 DEC 2001
  2. Article first published online: 25 DEC 2001
  3. Received 13 March 2000;accepted following revision 25 April 2000.

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Abstract

We have isolated a cDNA from Aedes aegypti that is transcribed in the larval midgut in response to metal exposure, and in the adult female midgut in response to iron or cadmium exposure, or a blood meal. The cDNA encodes a protein, designated Aedes aegypti intestinal mucin 1 (AEIMUC1), which has similarities with invertebrate intestinal mucins and peritrophins, and vertebrate mucins. Proline, serine and threonine comprise 30% of the amino acid composition of AEIMUC1, a characteristic of mucins. AEIMUC1 contains three cysteine-rich domains, two of which flank a proline/serine/threonine-rich domain, a feature shared by many mucin genes. This is the first report on the isolation of a metal-responsive gene from an aquatic insect.

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