Cloning and transcriptional expression of a leucokinin-like peptide receptor from the Southern cattle tick, Boophilus microplus (Acari: Ixodidae)
Article first published online: 25 DEC 2001
Insect Molecular Biology
Volume 9, Issue 5, pages 457–465, October 2000
How to Cite
Holmes, S. P., He, H., Chen, A. C., Lvie, G. W. and Pietrantonio, P. V. (2000), Cloning and transcriptional expression of a leucokinin-like peptide receptor from the Southern cattle tick, Boophilus microplus (Acari: Ixodidae). Insect Molecular Biology, 9: 457–465. doi: 10.1046/j.1365-2583.2000.00208.x
- Issue published online: 25 DEC 2001
- Article first published online: 25 DEC 2001
- Received 9 March 2000;accepted following revisions 30 May 2000.
- neuropeptide receptor;
- G-protein-coupled receptor;
- developmental expression;
- cattle fever tick;
- Drosophila melanogaster
Leucokinins are invertebrate neuropeptides that exhibit myotropic and diuretic activity. Only one leucokinin-like peptide receptor is known, the lymnokinin receptor from the mollusc Lymnaeastagnalis. A cDNA encoding a leucokinin-like peptide receptor was cloned from the Southern cattle tick, Boophilus microplus, a pest of cattle world-wide. This is the first neuropeptide receptor known from the Acari and the second known in the subfamily of leucokinin-like peptide G-protein-coupled receptors. The deduced amino acid sequence exhibits 40% identity to the lymnokinin receptor. The receptor transcript is present in all tick life stages as determined by semiquantitative reverse transcription polymerase chain reaction. We also propose that the sequence AAF50775.1 from the Drosophila melanogaster genome (CG10626) encodes the first identified insect leucokinin receptor.