Isolation of cDNA clones encoding putative odourant binding proteins from the antennae of the malaria-transmitting mosquito, Anopheles gambiae
Article first published online: 24 APR 2002
Insect Molecular Biology
Volume 11, Issue 2, pages 123–132, April 2002
How to Cite
Biessmann, H., Walter, M. F., Dimitratos, S. and Woods, D. (2002), Isolation of cDNA clones encoding putative odourant binding proteins from the antennae of the malaria-transmitting mosquito, Anopheles gambiae. Insect Molecular Biology, 11: 123–132. doi: 10.1046/j.1365-2583.2002.00316.x
- Issue published online: 24 APR 2002
- Article first published online: 24 APR 2002
- Received 28 August 2001; accepted after revision 26 October 2001.
- Anopheles gambiae;
- antennal cDNA libraries;
- odourant binding proteins;
- host finding
One way of controlling disease transmission by blood-feeding mosquitoes is to reduce the frequency of insect–host interaction, thus reducing the probability of parasite transmission and re-infection. A better understanding of the olfactory processes responsible for allowing mosquitoes to identify human hosts is required in order to develop methods that will interfere with host seeking. We have therefore initiated a molecular approach to isolate and characterize the genes and their products that are involved in the olfactory recognition pathway of the mosquito Anopheles gambiae, which is the main malaria vector in sub-Saharan Africa. We report here the isolation and preliminary characterization of several cDNAs from male and female A. gambiae antennal libraries that encode putative odourant binding proteins. Their conceptual translation products show extensive sequence similarity to known insect odourant binding proteins (OBPs)/pheromone binding proteins (PBPs), especially to those of D. melanogaster. The A. gambiae OBPs described here are expressed in the antennae of both genders, and some of the A. gambiae OBP genes are well conserved in other disease-transmitting mosquito species, such as Aedes aegypti and Culex quinquefasciatus.