Present address: Parasite and Vector Biology Division, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, UK.
Molecular analysis of multiple cytochrome P450 genes from the malaria vector, Anopheles gambiae
Article first published online: 13 SEP 2002
Insect Molecular Biology
Volume 11, Issue 5, pages 409–418, October 2002
How to Cite
Ranson, H., Nikou, D., Hutchinson, M., Wang, X., Roth, C. W., Hemingway, J. and Collins, F. H. (2002), Molecular analysis of multiple cytochrome P450 genes from the malaria vector, Anopheles gambiae. Insect Molecular Biology, 11: 409–418. doi: 10.1046/j.1365-2583.2002.00350.x
- Issue published online: 13 SEP 2002
- Article first published online: 13 SEP 2002
- Received 13 February 2002; accepted after revision 24 May 2002.
- insecticide resistance;
- polytene chromosomes;
- gene clusters
Cytochrome P450s are a superfamily of haemoproteins, important in the metabolism of endogenous compounds and xenobiotics. As a first step to elucidating the role of this family in insecticide resistance in the malaria mosquito, Anopheles gambiae, we have cloned and mapped multiple P450 genes. Sixteen cDNAs encoding full-length P450s were cloned and physically mapped to the mosquito's polytene chromosomes. Fourteen of these encode putative CYP6 proteins and two encode P450s belonging to the CYP9 class. Eighteen new A. gambiae Cyp4 P450 genes were identified using degenerate PCR primers, cDNAs were detected for ten and in situ locations for thirteen members of this gene family.