Cloning and characterization of a 70 kDa heat shock cognate gene (HSC70) from two species of Chironomus
Article first published online: 22 JAN 2003
Insect Molecular Biology
Volume 12, Issue 1, pages 19–26, February 2003
How to Cite
Karouna-Renier, N. K., Yang, W.-J. and Rao, K. R. (2003), Cloning and characterization of a 70 kDa heat shock cognate gene (HSC70) from two species of Chironomus. Insect Molecular Biology, 12: 19–26. doi: 10.1046/j.1365-2583.2003.00383.x
- Issue published online: 22 JAN 2003
- Article first published online: 22 JAN 2003
- Received 9 July 2002; accepted after revision 16 July 2002.
- stress proteins
In the present study we carried out the isolation and characterization of an HSC70 gene from two midges, Chironomus tentans and C. yoshimatsui. The HSC70 cDNAs are approximately 2424 (C. tentans) and 2464 bp (C. yoshimatsui) long, and contain 1950 and 1956 bp open reading frames, respectively. Analysis of genomic DNA revealed the presence of two introns in these genes. The 5′ untranslated regions of the HSC70 genes are adenosine-rich, a feature found in inducible HSP70 genes. The nucleotide and amino acid sequences exhibit high identity with cytosolic HSC70s from other Dipterans. Northern hybridization indicated that HSC70 is expressed at all developmental stages, from embryo to adult, and Southern hybridization confirmed the presence of multiple HSP70 genes in Chironomus.